Before you get too into creating contigs and aligning them all to each other, it is often really useful to run a base call on your freshly relieved sequences. But this is easily explained by CodonCode. Oddly enough, one of the most useful features of the software, comparing all the contigs you've assembled, needs to be added to the toolbar. If you are starting out with Codon, watch those videos! From that base you can start trouble shooting and finding your own ways to do things, which is made much easier with the wide range to tutorials available from the manufacturers. After checking out a few tutorial video on YouTube, I was able to do the basics such as import my sequences, create contigs based on names and visually inspect and correct these contigs. So I've recently been using the trial version of Codon to check out some Cyclopia sequences, and damn this program is easy to use once you know where and what the different functions do. ab1 files, normally have limited capabilities compared to their expensive counterparts such as CodonCode Aligner.
ADDING FEATURES TO SEQUECES IN CODONCODE ALIGNER SOFTWARE
Secondly, there are many ways of opening these files, however, freely available software options for handling your. These files contain an electropherogram as well as the DNA base sequence, whichĬan be viewed using a DNA viewer program.
ab1 files are are the output format for raw DNA data produced by Applied Biosystems' Sequencing Analysis ab1 files any way? I WANT MY SEQUENCES!!! The struggle is real! Image courtesy Sketching Science.Īfter hours (that felt like years) battling with DNA extraction methods and PCR protocols you have finally received the sacred e-mail, "please see attached sequences." and it all starts seeming worthwhile, maybe.īut now what, you've done the lab work but what are you supposed to do with these.
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